The heart is the first organ formed during the development of vertebrates. Requires timely and appropriate regulation of gene transcription . The sarcomere is the basic component of contractile muscle, and its formation is also a complex process, involving the participation of hundreds of genes, and requires precise assembly to form. Deletion of these genes results in cardiomyopathy, a major cause of morbidity and mortality worldwide. Although the function and structure of sarcomeres have been extensively studied, the exact process of sarcomere formation remains to be elucidated. However, the source of human body material has always been a bottleneck in the study of human gene operation. Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), with their ability to differentiate into all cell types, such as cardiomyocytes, are a powerful experimental model.
My laboratory mainly uses hPSCs as materials to study the mechanism of human heart development and heart disease. we are committed today

1. Use patient-specific induced pluripotent stem cells (iPSC) or CRISPR/Cas9 methods to generate isogenic cell lines to study cardiac cell development and construct disease models, such as dilated cardiomyopathy (DCM) and glycogen storage disease (GSD).

2. To explore the role of non-coding RNA including long non-coding RNA (lncRNA) or circular RNA in cardiac development and diseases.

3. Investigating the role of the 𝛾-tubulin protein complex in neural differentiation and disease.

The ultimate goals of our research are (1) to understand the process of human heart development, (2) to discover the pathophysiology of heart diseases, and (3) to identify potential therapeutic drugs for these diseases.